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b could be the dissociation continuous of the team that need to be protonated for complete activity. The pH profiles which confirmed the deprotonation of two acid ic groups along with the protonation of 1 basic team are required for max activity ended up in shape to equation 5 in which K

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For The one histidine substitutions, the N61H mutant is comparable in catalytic exercise on the double mutant whereas the Q59H mutant is diminished by lower than an buy of magnitude relative into the wild style enzyme. With the substitutions at Asn-sixty one, the alternative by having an alanine is noticeably a lot less disruptive compared to the adjust to the histidine.

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a worth is a little larger for Cd-NagA than for Zn-NagA, as expected from the inclination of zinc to decreased the p

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